Materials and Methods

3.1 Materials

3.1.1 Plant materials


Representative samples of A. paniculata collected from various regions in India and other countries of Asia were used for the study. Seeds from foreign sources were either collected (Malaysia) or received as gift. Four-month-old plants raised through seed propagation and reared under identical conditions served as the source of young leaf tissues used in various experiments. The plants collected from natural habitats and then grown in the garden campus were treated as ex situ populations (= germplasm accessions) while those taken from the natural habitats and directly used in various experiments were considered as in situ populations (= natural populations). For mutation and possible selection of high yielding variants, based on the yield performance a sample collected from Tamil Nadu (AP36) was used.


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